In standard muffin tin lined with paper liners layer two spoons of muffin batter, dollop cream cheese mixture in the center, top with remaining muffin batter and generously sprinkle with streusel crumbs. Then add dry ingredients to the wet and whisk to combine. In a large bowl whisk pumpkin, sugar and brown sugar, oil, eggs and milk. To make the pumpkin muffin batter in separate bowl whisk dry ingredients: flour, spices, baking powder and salt. In another bowl using a mixer beat softened cream cheese, sugar and vanilla for two minutes. In a fist bowl combine streusel ingredients: flour, brown sugar, cinnamon, dash of salt and melted butter and whisk with a fork. How To Make Pumpkin Cream Cheese Muffins: Whether you eat them warm, cold, or room temperature, try not to eat too many, if you can. They have a couple of steps, need four mixing bowls, but it’s worth it. you can also substitute milk if you don’t have cream You can substitute with pumpkin pie spice if you wish, but I personally like to make my own combo adjusted to my taste spices: cinnamon, nutmeg, ginger, cloves, all spice.brown sugar, granulated sugar and powdered sugar.You can use homemade puree or the one from the can pumpkin puree- make sure to use pure pumpkin not pumpkin pie filling.This easy muffin recipe that makes the best homemade muffins from scratch, requires only simple ingredients that you probably have on hand: Very easy to make, store and transport for sharing.They are topped with crunchy brown sugar cinnamon streusel topping-irresistible.They are filled with a cream cheese and taste like a combo of Pumpkin Coffee Cake and Pumpkin Cheesecake.Pumpkin flavor is perfectly complemented with cinnamon, nutmeg, ginger and cloves (you can substitute with pumpkin pie spice). ![]() Eating dessert for breakfast without filling guilt.They are the best fall breakfast loaded with spices.Why You’ll Love These Pumpkin Cheesecake Muffins: Luckily, season of comfortable and cozy clothes is upon us, because you won’t be able to eat just one of these. Seriously, what is not to love about this pumpkin perfection! Easy to make from scratch, these pumpkin cheesecake muffins are great for breakfast, brunch, snack, dessert, Halloween, Thanksgiving…any time! ![]() ![]() If you have your fall to-do list, you must add baking these muffins. The Best Pumpkin Cream Cheese Muffins From Scratch Combo of perfectly spiced, soft and moist pumpkin muffins, delicious cream cheese filling, crunchy brown sugar cinnamon streusel topping, and a sweet glaze sounds like the perfect fall comfort food. Pumpkin Cream Cheese Muffins are the best pumpkin muffin recipe ever.
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The development in the blotting techniques has helped provide scientists with a tool to study molecular interactions and detect changes, if any. The southern blot protocol begins with DNA. The two techniques stem from the same principle and differ in the type of molecules they target. Southern Blot Southern blots are used to determine the identity, size, and abundance of specific DNA sequences. Southwestern blotting studies that detect DNA-binding proteins. Northwestern blotting studies that detect interactions between RNA and proteins. To study genetic changes in DNA it can be used for homology-based cloning too. The membrane used is a nitrocellulose membrane. The membrane used is an amino benzyloxymethyl filter paper membrane. It detects changes in specific DNA sequences. It detects presence of specific RNA sequences. The name is a misnomer―an eponymous derivative of Southern blotting. It was developed by Sir Edwin Mellor Southern in 1975. It was developed by James Alwine, David Kemp, and George Stark in 1977. However, there are some cases where one has to use radioactive labeling. Nowadays, fluorescent dyes are preferred over radioactive labeling to prevent undue exposure to radiation. If a chromogenic dye is used, the results can be seen on the membrane itself. In case of radioactive labeling or the use of a fluorescent dye, an X-ray film is exposed to the membrane for a few seconds and developed. ✦ Depending on the type of secondary probe used, the membrane is visualized accordingly. The membrane is washed gently to eliminate the excess probes, and then it undergoes secondary hybridization, where the target probe is labeled with a radioactive element, a fluorescent dye, or even a chromogenic dye. The binding of the probe to the target molecule is called primary hybridization. The membrane is then treated with specific target probes that bind to the transferred bands. ✦ Once the blocking is done, the membrane is washed gently to remove traces of the blocking solution. This ensures that the transfer is made permanent, and the unbound surface of the membrane is blocked so as to prevent binding of unwanted molecules and the possibility of a hampered result. The membrane with the transferred bands is then sealed by baking in an oven or by treating it with a blocking solution. The bands are then transferred onto a carrier membrane by the principle of either capillary action or directed electric current. ✦ Initially, the purified sample is loaded onto an agarose gel and electrophoresed, causing the sample to separate into bands. The intensity of the resultant dye or fluorescence will indicate the specificity of the binding along with other characteristics such as copy number, gene expression, etc. This change in binding affinity will be visualized upon secondary hybridization with a labeled probe. Any change in the sample sequences will lead to the probes not binding or binding non-specifically. PrincipleĪll blotting techniques are based on the same principle of specific base pairing of the probe sequences to the sequences immobilized on the membrane. The purpose of each technique may be different, but all share the same principle and methodology, with a few minor deviations and modifications. Southern blotting is used in case of DNA, Northern blotting in case of RNA, Western blotting in case of proteins, and Eastern blotting in case of post-translational modifications of proteins. In molecular biology and genetics, various blotting techniques are employed to detect and study changing levels of proteins, DNA, or RNA, and also to study the interactions occurring between them. Subsequent blotting techniques (Northern blotting, Western blotting, etc.), which are based on the same principle, are named eponymously. Southern, a British biologist who developed this technique. This online MCAT prep course, offers the best audio learning experience and is a great addition to our MCAT Prep App.Southern blotting is named after Sir E. MCAT Go is browser based which means you can access it on any device. Western blot is for separating proteins and this can also be used to quantify gene expression. This is often used to quantify gene expression. Northern blot is for separating RNA molecules. This is often used for genotyping different organisms. ![]() Now real quick southern blot is used to separate DNA. So you can see the Snow… Drop for memorizing these molecular biology techniques. SNOW DROP is for helping you to memorize the different types of blotting techniques on the MCAT. Today we have an MCAT biology mnemonic for you, and it’s for molecular biology techniques. My name is Ken and I’m an MCAT expert with MedSchoolCoach. Welcome back to another MCAT Mnemonic Monday. Ken Tao is the MedSchoolCoach expert on MCAT, and helps you remember Southern blot, Northern blot, and Western blot with the acronym: Snow Drop. ![]() Weakly Director, Dairy Forage Nutrition Research Forage Genetics, West Salem, WI Forage Storage: Life after Harvest ( PDF) Dr. Officials with the World Dairy Expo confirmed Thursday that Dane County’s largest convention and exposition will once again be held at the Alliant Energy Center this fall continuing a more than 50-year tradition that was disrupted last year by the COVID-19 pandemic. LEAF (Leaves Enhance Alfalfa Forage) Testing Provides Opportunities for Improving Alfalfa Quality Dr. Designed for dairy producers and industry partners World Dairy Expo is a showcase for elite dairy cattle, cutting edge research and modern technologies. Bernard Professor Emeritus University of Georgia, Tifton, GA. No other dairy event in the world compares. Download the World Dairy Expo mobile event app, visit or follow WDE on Facebook, Twitter, LinkedIn, Spotify, Instagram or YouTube for more information.World Dairy Expo is where the dairy industry meets. The fundraiser is for the Wisconsin State Fair Dairy Promotion Board. Admission tickets to WDE can be purchased at . The 2023 Grand Master Cheesemaker, Grand Champion Butter, Grand Champion Yogurt, Grand Champion Sour Cream, Grand Champion Milk and Grand Champion Custard will be announced at the Blue Ribbon Dairy Products Auction on Thursday, August 10 at The Wisconsin State Fair Park. for the 55 th event, October 2-7, 2022, when the world’s largest dairy-focused trade show, dairy and forage seminars, a world-class dairy cattle show and more will be on display. The global dairy industry will return to Madison, Wis. Taking Reserve Junior Champion honors was the first-place Spring Yearling, Spots Pride Play It Again, owned by Tristen Ostrom of Kaukauna, Wisconsin.Įvaluating the 154 entries in the International Junior Brown Swiss Show were official judge Gerrit DeBruin of Lake Mills, Wisconsin and associate judge Hayden Hauschildt of Ellsworth, Wisconsin.Ĭomplete class results can be found at Serving as the meeting place of the global dairy industry, World Dairy Expo brings together the latest in dairy innovation and the best cattle in North America. Perfection is owned by Jacob Harbaugh and Matthew Thompson of Marion, Wisconsin. The winning Summer Yearling, Random Luck Total Perfection, was the last heifer standing as she was named Junior Champion of the International Junior Brown Swiss Show. Rounding out the champion cows was the winning Senior Two-Year-Old and Reserve Intermediate Champion, Terra Rose Cliff Shasta, exhibited by Adele Biasini of Morrisville, Vermont. The 55th annual event runs through Friday at Alliant Energy Center. Kruses LJ Fab Skylar-ET, owned by Cole & Carter Kruse of Dyersville, Iowa, was the winning Junior Three-Year-Old and Intermediate Champion of the International Junior Brown Swiss Show. Elizabeth Kiko of R&P Kiko Farm in Salem, Ohio, reacts after winning the Junior Champion Female award in the International Holstein Show with her winter yearling, Jawdropping, during Monday's competition at World Dairy Expo. Crowds of more than 62,000 people, from nearly 100 countries, will return to Madison, Wis. The contest yielded several winners from Wisconsin. Tea Rose, owned by Matthew & Allison Thompson of Darlington, Wisconsin, took home the Lillian & Keith King and Jim King Reserve Grand Champion of the Junior Show Award. 0 Greg Nielsen scoops filling from an ice cream sandwich while judging the healthy frozen desserts category during last month's World Dairy Expo Championship Dairy Product Contest at Madison Area Technical College in Madison. The 54 th edition of the event is scheduled for Expo’s historic home, the Alliant Energy Center, September 28 October 2, 2021. Random Luck B Tea Rose won Reserve Grand Champion after topping the Component Merit Cow Class and being named Reserve Senior Champion. World Dairy Expo is pleased to announce, after thorough consideration, World Dairy Expo 2021 will remain in Madison, Wisconsin. Groovy was exhibited by Hannah & Mark Balthaser of Bernville, Pennsylvania. This Senior Champion and winner of the Five-Year-Old Cow Class also took home the $500 Udder Comfort Grand Champion Cash Award and the Lillian & Keith King and Jim King Grand Champion of the Junior Show Award. MADISON, WIS. – Northkill Creek Groovy took home the 2022 title of Grand Champion of the International Junior Brown Swiss Show at World Dairy Expo.
![]() From the PlayStation 5 menu, you can see which multiplayer matches are available or what single-player missions you are on in the game. Players to see what is happening in a game without even booting it up. The PlayStation 5’s user interface is being expanded in innovative ways, one allowing Meaning one could get a little harder to push as you draw a bow. The controller also boasts new adaptive triggers that give developers the freedom to determine how much resistance is applied to the L2 and R2 buttons. You’ll feel the difference in textures as your car drives over rocks or mud through the haptic response. The PlayStation 5 controller replaces the rumble technology found in the DualShock 4 with haptic feedback, which will give players a wider range of feedback. ![]() The PlayStation 5 will feature a solid-state drive, an enhanced GPU capable of delivering ray tracing, and an 8-core, 16-thread CPU based on AMD’s Zen 2 microarchitecture. Here’s a quick description for some of the most exciting Sony PlayStation 5 console features from the experts at Game Informer magazine: PLAYSTATION 5 - FROM THE DESK OF GAME INFORMER Through your headphones or TV speakers your surroundings truly come alive with Tempest 3D AudioTech in supported games. Tempest 3D AudioTech - Immerse yourself in soundscapes where it feels as if the sound comes from every direction.Up to 8K Output - PS5™ consoles support 8K Output, so you can play games on your 4320p resolution display.Up to 120fps with 120Hz output - Enjoy smooth and fluid high frame rate gameplay at up to 120fps for compatible games, with support for 120Hz output on 4K displays.Ray Tracing - Immerse yourself in worlds with a new level of realism as rays of light are individually simulated, creating true-to-life shadows and reflections in supported PS5™ games.Integrated I/O The custom integration of the PS5™ console's systems lets creators pull data from the SSD so quickly that they can design games in ways never before possible.Ultra-High Speed SSD Maximize your play sessions with near instant load times for installed PS5™ games. Breathtaking Immersion - Discover a deeper gaming experience with support for haptic feedback, adaptive triggers, and 3D Audio technology.Lightning Speed - Harness the power of a custom CPU, GPU, and SSD with Integrated I/O that rewrite the rules of what a PlayStation® console can do.Some of the most exciting features of the PlayStation 5 include: With two different console models available, the PlayStation 5 (with physical drive) and the PlayStation 5 Digital Edition, gamers now have their choice between using physical discs or talking the all-digital route. The PS5 has brought true next-gen gaming to life, with stunning 4K graphics, silky-smooth framerates and a controller, the PlayStation 5 DualSense controller, that creates unparalleled immersion with new features, such as haptic feedback and adaptive triggers. The Sony PlayStation 5 console has finally arrived, and after much anticipation and hype, it’s safe to say the PlayStation 5 has absolutely delivered. To save the file on your device or to iCloud Drive, click Save, choose a location, then click Export.Choose a format, then choose any additional options for that format.Click Share in the toolbar, then click Export and Send.To save the file on your device or to iCloud Drive, click Save, then choose a location.To share the file, click Send a Copy, then choose how you want to send your file, such as with Mail or Messages.Choose File > Export To, then select the format.Choose how you want to send your file, such as with Mail or Messages, then send the file.Īfter you’ve exported the file, you can send the exported file with Mail, Messages, AirDrop or another available app, or save the file on your Mac or iCloud Drive. Open the Pages, Numbers or Keynote file you want to export.Choose a format, choose any additional options for that format, then tap Export. ![]() Tap the Share button in the toolbar, then tap Export and Send.You can also export and send or save a file using the Share menu in the toolbar: To save the file on your device or to iCloud Drive, choose Save to Files, then choose a location.Choose how you want to send your file, such as with Mail or Messages, then send the file.If the Export Details window appears, which shows what may be different in the exported version of the file, tap Continue. ![]() ![]() Select any additional options, then tap Export.You can also export to PDF and a variety of other file formats depending on the iWork app you’re using.To open a Keynote presentation in PowerPoint, choose PowerPoint.To open a Numbers spreadsheet in Excel, choose Excel.To open a Pages document in Word, choose Word. Level 1 9 points 2:13 PM in response to lecturer275 I have the very same problem, and solved it this way: 1) Open 'Print' from the File menu in Keynote, then choose 'PDF' and 'Save PDF as postscript' 2) Convert the ps to pdf (I use Acrobat Distiller) Colors are preserved for me.If you don't see the document name, tap the More button. Open the Pages, Numbers or Keynote file you want to export.You can do this either by exporting the presentation from Keynote to a different format, or by using a conversion tool.After you’ve exported the file, you can send the exported file with Mail, Messages, AirDrop or another available app, or save the file on your device or iCloud Drive. The files can't be opened by other presentation programs like PowerPoint or Google Slides, so to view the files on a Windows or Android device you would first need to convert the KEY files to another format like PDF or PPTX. However, the drawback with KEY files is that you need an Apple device to view and edit them. Apple users often use the in-built app because it’s available across their devices without extra downloads or subscriptions. You can also play a KEY presentation on a screen while reading the notes on your device.Īlong with Numbers and Pages, Keynote is installed as standard on iOS, iPadOS and macOS as part of the iWork suite. KEY presentations can be created from blank slides or from templates provided within the application. Keynote is an alternative to presentation software like PowerPoint and Prezi, and includes functions like layout templates, animations and embedded videos. KEY is the name of the format for files created by Apple’s Keynote presentation app. PowerPoint is part of the Microsoft Office suite, but some other programs, like LibreOffice, Apple Keynote or Google Slides, can also open both PPT and PPTX files. However, even if you have a newer version of the program, you can still use it to open older PPT files, and also to save presentations into PPT format. Since 2007, PPTX has been Microsoft’s standard file format for PowerPoint. It can also be used for creating reports, which users often save or print as a PDF. ![]() PowerPoint is the most widely used format for presentations in both educational and work settings. Users can build presentations from scratch or use an online or company corporate template. PPT files normally comprise slides of text, images and animations that can play as timed or manually controlled slideshows. Microsoft developed the PPT format for PowerPoint, which is mostly used for creating and editing presentations. ![]() With unique features for illustrators and sequential artists, as well as thriving communities, each software offers users a valuable experience and cost-effective entry into the digital-art sphere. There are a variety of PaintTool SAI alternatives such as ClipStudio Paint, Procreate, GIMP, Krita, and Medibang Paint amongst others. ![]() There are also helpful drawing tutorials utilizing the software with topics related to effects, coloring, and more. On the MediBang Paint website, users have access to a variety of downloadable custom materials such as brushes, screen tones, and templates. MediBang Paintĭeveloped in 2014, MediBang Paint (formerly known as CloudAlpaca) is a free, open-source digital painting software.Ĭompatible with Windows, Mac, and Android, MediBang paint is a great beginner software alternative to PaintTool SAI, with a robust and helpful community of artists surrounding the program. Available for Windows, Mac, Linux, and Chrome, it is a great introductory software for beginner artists. With vector text options, it surpasses PaintTool SAI in function and capability with a zero-dollar price point. Krita is a value software with a variety of functions perfect for creating simple animations, repeat patterns, webcomics, and more. Krita was developed by the Krita Foundation in 2005. Like PaintTool SAI, it is a choice software for illustrators and artists alike, with a flexible interface and custom brush settings. Like GIMP, Krita is also a FREE, open-source digital painting and photo editing software. This is perfect for an illustrator who combines photography, illustration, and animation in their work. Gimp also includes some simple animation functions to create animated GIFs. It has an easy-to-use intuitive interface, especially for users who are previously familiar with Photoshop.Īlthough the software’s primary focus is photo manipulation, there are a couple of notable illustrators who use it for their work, such as ctchrysler. GIMP is a free, open-source digital painting and editing software developed by the GIMP Development Team, and is available to download for Windows, Mac, and Linux users. The best part about GIMP is that it’s FREE! Yes, Free. GIMPĪnother popular drawing software alternative to PaintTool SAI is GIMP. Compared to PaintTool SAI’s price of approximately $52 USD, this is cheap. You can get Procreate exclusively in the Apple Store for a one-time payment of $12.99. It also comes with integrated special effects, a feature lacking in PaintTool SAI. ![]() With unique functions such as QuickShape and Color Drop, Procreate also offers users access to a variety of workflow-optimizing functions, as well as a large asset library of custom brushes. Commonly used on iPad Pro by most users, Procreate is the best PaintTool SAI alternative for tablet artists.Īs PaintTool SAI is currently only available on Windows, Procreate is more suited if you wish to draw on the go instead of being tied to a computer or laptop screen. Developed by Savage Interactive, Procreate is a raster-based digital painting and editing software compatible with iOS and iPadOS. ProcreateĪnother Alternative to PaintTool SAI and a favorite amongst illustrators is Procreate. It is powerful software that has a learning curve to master but offers its users an active and dynamic community with an ever-growing asset library for custom brushes, stamps, 3D models, animation effects, etc. However, you can also pay by monthly plan starting at $0.99, or buy a license of Clip Studio Paint Pro for $219.00.Ĭompared to PaintTool SAI, Clip Studio is preferred by webcomic and sequential artists due to its native features optimized for text placement, integrated 3D models, animation, and more. It is the closest to PaintTool SAI in price point, with a single license of Clip Studio Paint Pro costing $49.99. Clip Studio Paint, previously known as Manga Studio is a digital drawing software distributed by the Japanese company Celsys. |